Abcam, ab136716, Rabbit Anti-Rat IgG H&L (beta-galactosidase)

Size: 500µL
Suitable for WB, ICC, IHC-Fr, IHC-P, ELISA. Cited in 2 publications.
Key facts
Host species:Rabbit,
Target species:Rat,
Target isotype:IgG,
Target specificity:Heavy & Light chains,
Pre-adsorbed:No,
Conjugation:Beta-galactosidase,
Applications:WB, ICC, IHC-Fr, IHC-P, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Clonality:Polyclonal,
Isotype:IgG,
Specificity:Beta-galactosidase labelling is well adapted to ELISA and produces no or very little background. It is more difficult to use in immunochemistry where its use can be justified only when the endogenous activities of other available labels produce significant problems.

Product details:
Preparation of Substrate Solutions: 0.1M phosphate buffer pH7, 1mM MgSO
, 0.2mM MnSO
, 2mM Mg titriplex. Add extemporaneously 7µl/ml beta-mercaptoethanol and 0.9mg/ml ortho-nitrophenyl-beta-galactopyranoside. Incubate for 30 minutes at 37°C. Stop the reaction with V/2 volume of 1M Na

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab136716 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography)., Storage buffer-Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Avoid freeze / thaw cycle