Abcam, ab136764, Rabbit F(ab')2 Anti-Dog IgG H&L (Alkaline Phosphatase)

Size: 500µL
Suitable for FuncS, ELISA, IHC-P, WB, IHC-Fr, ICC.
Key facts
Host species:Rabbit,
Target species:Dog,
Target isotype:IgG,
Target specificity:Heavy & Light chains,
Pre-adsorbed:No,
Conjugation:Alkaline Phosphatase,
Applications:IHC-Fr, FuncS, ICC, IHC-P, WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Clonality:Polyclonal,
Isotype:IgG

Product details:
Preparation of Substrate Solutions:
ELISA : 1M diethanolamin, 0.5mM MgCl
, 15mM pNPP, pH 9.8. Incubate for 15 minutes at 37°C. Stop the reaction with V/5 K
2M. Read at 405 nm.
Immunoblot : 34 µl of BCIP (5bromo-4chloroindolylphosphate) at 50mg/ml in DMF + 44 µl NBT (NitroBlue Tetrazolium) at 75 mg/ml in DMF 70% + 10ml substrate buffer (0.1M TRIS/HCI, 0.5M NaCl, 0.05M MgCl
, pH 9.5).

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab136764 is purified by affinity chromatography and is thus particularly specific., Storage buffer-Preservative: 0.1% Sodium azideConstituents: 50% Glycerol (glycerin, glycerine), 48.9% Tris buffered saline, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle