Abcam, ab136806, HeLa Apoptosis Lysate Set: Staurosporine-Treated and Vehicle-Treated Control

Size: 2Unit
Staurosporine is a broad-spectrum inhibitor of serine/threonine kinases and tyrosine kinases.
Product details:
Staurosporine is a broad-spectrum inhibitor of serine/threonine kinases and tyrosine kinases. It is commonly used to induce apoptosis in experimental settings, although the mechanism is not fully understood.
The staurosporine-treated HeLa lysate is designed for use as a western blot positive control for antibodies against apoptosis-specific proteins or for experimental treatments to induce apoptosis.
Confluent HeLa cells were treated with either the vehicle (DMSO, 0.2%) or staurosporine (1 uM) for 4 hours. Cells were pelleted then lysed with Lysis Buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10 mM NaF, 1x Protease Inhibitors, 1% SDS) and the lysate cleared by sonication. The protein concentration was measured by BCA assay. Modified 5x SDS sample buffer was added to the lysate before storage at -80°C. The lysates were validated by western blotting with the
ab136812
Apoptosis WB cocktail (cleaved PARP, actin, caspase 3).

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information--80°C