Abcam, ab136916, Anti-C3d antibody [E28-P]

Size: 250µL
Rabbit Monoclonal C3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human C3.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:E28-P,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human C3. The exact immunogen used to generate this antibody is proprietary information.P01024,
Epitope:Peptide derived from N-terminal sequence of human C3d complement fragment,
Specificity:This product is specific to C3d, but also C3b and iC3b, since C3d is a product from C3b.

Properties and Storage Information:
Form-Liquid, Purification technique-Proprietary technique, Purification notes-This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a proprietary technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability., Storage buffer-pH: 8Preservative: 0.05% Sodium azideConstituents: 2% BSA, 0.32% Tris HCl, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C3d a fragment of the complement component C3 plays an important role in the immune response. It weighs around 35 kDa. This protein fragment forms after the cleavage of C3 and is found in the bloodstream. Researchers often refer to C3d in the context of complement activation using terms such as 'C3d complement' and 'anti-C3d-positive'. C3d is expressed extensively on cell surfaces where it binds to receptors modulating immune cell responses.
Biological function summary
The fragment plays a significant role in the regulation of immune activation processes. C3d is part of the complement system which is a complex network of proteins contributing to innate immunity. This fragment tags pathogens for destruction and acts as a bridge for enhancing adaptive immunity by linking innate and adaptive immune responses. Interaction with the complement receptor type 2 (CR2) on B cells and follicular dendritic cells is essential for its immunological function.
Pathways
C3d participates in the complement activation pathway and is an important player in the alternative pathway of complement activation. Through these pathways it is linked to proteins such as Factor B and Factor D further influencing the cascade leading to pathogen elimination. The amplification loop involving C3 and its fragments including C3d enhances the immune response emphasizing its connectivity within these pathways.
The role of C3d becomes particularly relevant in conditions like systemic lupus erythematosus (SLE) and age-related macular degeneration (AMD). Abnormal deposition of C3d and its fragments associates with inflammatory disorders such as SLE. Connection to CR2 is significant as the CR2-C3d complex can exacerbate these diseases. Understanding these interactions helps in targeting therapies for related autoimmune and inflammatory conditions.