Abcam, ab137102, Anti-dUTPase antibody [EPR9607(B)]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal dUTPase antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR9607(B),
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
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Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The enzyme deoxyuridine triphosphatese also known as dUTPase hydrolyzes dUTP to dUMP and inorganic pyrophosphate. It prevents the incorporation of uracil into DNA which can lead to mutations. The molecular mass of human dUTPase is about 18-20 kDa. It is widely expressed in both prokaryotic and eukaryotic cells including places like the thymus and spleen where DNA synthesis is very active. Alternative names such as DUT or dUTP pyrophosphatase are often used to refer to this enzyme.
Biological function summary
DUTPase maintains nucleotide pool balance and genome integrity. By converting dUTP to dUMP it provides substrates for thymidylate synthase to produce thymidine triphosphate (dTTP) which is essential for DNA replication and repair. dUTPase is not typically part of a larger protein complex but cooperates with other enzymes in nucleotide metabolism. Its activity protects cells from apoptosis that might occur due to extensive uracil misincorporation.
Pathways
DUTPase plays a role in nucleotide metabolism and one-carbon metabolism pathways. It regulates the pyrimidine metabolism pathway ensuring sufficient dTTP levels for DNA synthesis. dUTPase interacts indirectly with proteins like thymidylate synthase and dihydrofolate reductase through these pathways. These interactions aid in maintaining a steady supply of nucleotides important for DNA replication and repair.
Impairments in dUTPase function can relate to cancer and immunodeficiency disorders. In some cancers altered dUTPase expression affects the sensitivity to chemotherapeutic agents particularly those targeting thymidylate synthesis. In certain immunodeficiencies dUTPase malfunction leads to increased uracil incorporation compromising DNA integrity. The enzyme connects with proteins such as thymidylate synthase in these diseases due to its role in managing nucleotide pools critical for cell proliferation.