Abcam, ab13723, Anti-SMAD 1/5/8/9 antibody

Size: 100µg
Rabbit Polyclonal SMAD1 antibody. Suitable for WB and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD1 aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SMAD1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.Q15797,
Specificity:ab13723 may cross-react with SMAD2 or SMAD3. The actual SMADs detected with this antibody may vary according to cell or tissue-type specific expression

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SMAD 1/5/8/9 also known by the genes SMAD1 SMAD5 SMAD8 and SMAD9 are integral proteins in the transforming growth factor-beta (TGF-β) signaling pathway. These proteins function as transcriptional modulators once activated. The molecular weight of SMAD1 SMAD5 and SMAD8 ranges from approximately 60 to 65 kDa. These proteins are expressed in various tissues and are especially active in bone cartilage and the vascular system. They act by transmitting extracellular signals from TGF-β superfamily ligands to the nucleus leading to gene expression changes.
Biological function summary
SMAD1/5/8/9 proteins play essential roles in processes like cellular proliferation differentiation and apoptosis. These proteins typically form complexes with other SMAD proteins such as SMAD4 upon activation by type I receptor kinases. The formation of these complexes is important for their translocation to the nucleus where they regulate target gene expression. This regulation is key in controlling pivotal developmental and homeostatic processes across different cell types.
Pathways
SMAD1/5/8/9 proteins are central components of the bone morphogenetic protein (BMP) signaling pathway. They mediate the signal transduction from BMPs which are important for bone development and embryogenesis. These proteins interact with other signaling molecules such as BMP receptors and various co-SMADs like SMAD4 to relay and modulate signals effectively. Through these interactions SMAD1/5/8/9 function within a well-defined network ensuring precise cellular responses to BMP stimuli.
SMAD1/5/8/9 proteins show significant involvement in conditions like cancer and pulmonary arterial hypertension (PAH). Aberrant BMP signaling through dysfunctions in these SMAD proteins has been linked to tumorigenesis where abnormal cell proliferation is a hallmark. Additionally alterations in SMAD1/5/8/9 activity are associated with PAH a condition characterized by high blood pressure in the pulmonary arteries. In these diseases SMAD4 often acts as a partner in complex formation with SMAD1/5/8/9 underlining its importance in maintaining normal signaling processes.