Abcam, ab137989, Human Protein Z ELISA Kit

Size: 1 x 96Tests
Human Protein Z ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Protein Z in Human in Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum, Milk, Cerebral Spinal Fluid, Urine,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:> 64 pg/mL,
Range:0.098 - 25 ng/mL,
Assay time:4h,
Assay Platform:Microplate

Product details:
Abcam's Protein Z Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Protein Z in plasma, serum, urine, milk, and cell culture supernatants.
A Protein Z specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Protein Z specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Protein Z captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein Z also known as PZ is a vitamin K-dependent plasma protein with a molecular mass of approximately 62 kDa. It is primarily expressed in the liver and circulates in the blood. This protein plays a mechanical role in the coagulation system by interacting with the protein Z-dependent protease inhibitor which downregulates blood coagulation contributing to hemostasis. Protein Z is different from other coagulation proteins due to its lack of enzymatic activity but impacts the regulation of thrombin formation.
Biological function summary
Protein Z serves as a significant factor in the coagulation cascade being a part of the protein C pathway complex. Although it lacks enzymatic action Protein Z interacts with other proteins such as Factor Xa and Protein Z-dependent protease inhibitor to exert its effects. Its involvement helps modulate clot formation and stabilize the coagulation process impacting blood flow regulation.
Pathways
Protein Z is intricately linked to the coagulation and thrombosis pathways. It associates with proteins including Factor Xa and Protein C. Protein Z assists in regulating thrombin generation by inhibiting excess coagulation maintaining a balance within the hemostatic system. This interaction ensures the rapid and effective stopping of blood flow when required while preventing unwarranted thrombosis a critical aspect of circulatory system maintenance.
Protein Z levels link to thrombosis and hemorrhagic disorders. Abnormalities in Protein Z levels have connections with deep vein thrombosis due to its role in regulating coagulation balance. The protein Z-dependent protease inhibitor's association highlights its significance in clot formation and thrombosis prevention. Additionally deficiencies in Protein Z have connections with bleeding disorders emphasizing the protein's role in maintaining a balanced coagulation process essential for preventing excessive bleeding or clotting.