Abcam, ab139413, Plasma Membrane Fraction Western Blot Cocktail

Size: 200µL
Plasma Membrane Fraction Western Blot Cocktail (ab139413) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Key facts
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Reacts with:Mouse, Rat, Human,
Form:LiquidSee storage information,
Storage buffer:Preservative: 0.02% Sodium azideConstituents: 0.61% Tris, 0.5% BSA, 0.015% Dodecyl maltoside

Product details:
ab139413 contains 3 mouse monoclonal antibodies each targeting a specific organelle marker. The presence of plasma membrane is determined by Anti-Sodium Potassium ATPase antibody; cytosol by Anti-GAPDH; and nucleus by Anti-Histone H3 (di methyl K9). This cocktail is suitable for determining the purity of organelle isolates prior to further characterization.
This product is particularly valuable to researchers working in organelle proteomics. Mass spectrometry is frequently used in this field to determine the protein content of targeted organelle isolates. These isolates are obtained using differential centrifugation, density gradient fractionation, biochemical enrichment, or affinity purification. Unfortunately, the various methods of purification available for organelle isolation are imperfect and leave behind contaminants from undesired regions of the cell. These contaminants are inevitable, but being aware of which contaminants are present is crucial for analysis of mass spectrometry results. The high sensitivity and species cross reactivity of the antibodies in this cocktail will quickly and easily reveal impurities caused by imperfect sample preparation.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Sodium Potassium ATPase also known as Na+/K+ pump is a vital membrane protein with an approximate molecular mass of 112 kDa. It is expressed in almost every cell type across various tissues regulating the maintenance of intracellular ion gradients. Mechanically it pumps three sodium ions out of the cell and two potassium ions into the cell against their concentration gradients using ATP hydrolysis therefore maintaining electrochemical gradients essential for numerous cellular processes.
Biological function summary
This ATPase plays a central role in cellular osmotic balance and volume regulation. It operates as a part of a larger enzyme complex that interacts with other proteins to ensure proper cell function. Besides its key involvement in ion transport this enzyme influences cellular processes like signal transduction and nutrient uptake by modulating membrane potential and cell responses.
Pathways
Sodium Potassium ATPase is integrated into vital signaling pathways that include the MAPK/ERK and PKA pathways. Through these pathways it interacts with proteins such as growth factors and neurotransmitter receptors impacting cell proliferation and communication. This integration highlights its importance in maintaining cellular homeostasis and facilitating intercellular communication.
Sodium Potassium ATPase dysregulation associates with conditions like congestive heart failure and hypertension. Its impaired activity can affect cardiac muscle contraction and blood pressure regulation. Additionally alterations in its function have links to proteins like ankyrin which may further exacerbate its role in cardiovascular diseases emphasizing the need for understanding this target in therapeutic contexts.