Abcam, ab139615, Recombinant human STK36 protein

Size: 10µg
Recombinant human STK36 protein is a Human Fragment protein, in the 1 to 457 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, WB, FuncS.
Key facts
Purity:>80% Densitometry,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:FuncS, WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The specific activity of ab139615 was determined to be 0.6 nmol/min/mg.,
Accession:Q9NRP7,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Product details:
ab64311
(Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
STK36 also known as serine/threonine kinase 36 is a protein with a molecular weight of approximately 134 kDa. It functions primarily as a kinase transferring phosphate groups from ATP to serine or threonine residues on target proteins. STK36 is expressed in various tissues with higher expression levels observed in the brain and testis. This expression suggests a significant role in cellular processes in these areas. STK36's activity is important for maintaining proper signaling pathways within cells.
Biological function summary
STK36 plays an essential role in the sonic hedgehog (SHH) signaling pathway which regulates embryonic development and cell differentiation. It acts as part of a complex that includes GLI transcription factors influencing their activity through phosphorylation. By regulating the activation of GLI proteins STK36 affects the transcription of genes involved in growth and morphogenesis. This regulatory mechanism highlights its influence on cellular development and division.
Pathways
The protein interacts mainly within the SHH signaling axis. It stabilizes the activity of the GLI transcription factors important for the expression of genes like PTCH1 and BCL2. Additionally STK36 operates within the context of the primary cilia a cellular structure where it works alongside proteins such as Smoothened (SMO) to mediate signal transduction. Through its pathway interactions STK36 directly affects the development and function of various cellular processes.
Mutations or dysregulation of STK36 have been linked to conditions such as medulloblastoma and hydrocephalus. In these contexts its interactions with proteins like the Suppressor of Fused (SUFU) become disrupted leading to aberrant SHH pathway activity. These diseases highlight the importance of STK36 in maintaining normal cellular signaling illustrating how its disruption can have profound pathological effects.