Abcam, ab139726, Anti-LMX1A antibody - C-terminal

Size: 100µg
Anti-LMX1A antibody - C-terminal (ab139726) is a rabbit polyclonal antibody detecting LMX1A in Western Blot, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Over 10 publications
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human LMX1A aa 300-350. The exact immunogen used to generate this antibody is proprietary information.Q8TE12,
Specificity:At least two isoforms are known to exist. ab139726 is predicted to not cross-react with LMX1B.

Product details:
What is this antibody validated in?
Anti-LMX1A antibody - C-terminal (ab139726) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of LMX1A?
Anti-LMX1A - C-terminal (ab139726) specifically detects a band for LMX1A (UniProt: Q8TE12) at a molecular weight of 42kDa.
Trusted by the scientific community
Anti-LMX1A - C-terminal (ab139726) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LMX1A also known as LIM homeobox transcription factor 1 alpha serves as a transcription factor involved in embryonic development. It belongs to the LIM homeobox gene family and weighs approximately 42 kDa. This protein primarily localizes in the central nervous system and developing limb tissues. It plays an important role in the differentiation and development of dopamine-producing neurons which are important for proper nervous system function.
Biological function summary
The presence of LMX1A is essential for the development of dopaminergic neurons in the midbrain. It functions as part of a complex transcriptional network with other key transcription factors such as NURR1 and PITX3 to regulate genes essential for dopamine synthesis and neuron maintenance. Through its actions LMX1A influences the dopaminergic system contributing to motor control and behavior regulation. This makes it important for studies in neurobiology and developmental biology.
Pathways
LMX1A is an integral component in the Wnt signaling pathway which is vital for maintaining dopaminergic neuron populations. It directly impacts the regulation and expression of genes related to dopamine synthesis. In collaboration with transcription factors like NURR1 LMX1A promotes the production of enzymes necessary for dopamine metabolism. This pathway plays an important role in neuronal survival and connectivity illustrating the connection of LMX1A to other proteins within this system.
Alterations in LMX1A expression or function are linked to Parkinson's disease and neural developmental disorders. In Parkinson's disease the degeneration of dopaminergic neurons occurs where LMX1A plays an important role in neuronal development and maintenance showcasing its potential therapeutic significance. Furthermore downregulation of LMX1A can lead to a disrupted neuronal development illustrating its impact on diseases associated with neurological development while the protein NURR1 is also critically involved in these processes.