Abcam, ab140171, Anti-PMPCA/INPP5E antibody

Size: 100µg
Rabbit Polyclonal PMPCA/INPP5E antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PMPCA aa 150-400.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PMPCA aa 150-400. The exact immunogen used to generate this antibody is proprietary information.Q10713

Properties and Storage Information:
Form-Lyophilized, Reconstitution-Reconstitute in 200 µL of water, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PMPCA (peptidase (mitochondrial processing) beta) and INPP5E (inositol polyphosphate-5-phosphatase E) are proteins involved in distinct cellular functions. PMPCA is a subunit of a mitochondrial processing peptidase that weighs approximately 56 kDa. It is primarily expressed in the mitochondria where it processes precursor proteins by cleaving specific N-terminal sequences to facilitate proper protein maturation. INPP5E is a lipid phosphatase localized to cilia and cytoplasm with a mass of about 72 kDa playing a role in phosphoinositide signaling pathways.
Biological function summary
The proteins perform unique functions essential to cell homeostasis and function. PMPCA plays a role in mitochondrial protein maintenance and is necessary for mitochondrial integrity. INPP5E engages in phosphoinositide metabolism and is part of a multi-protein complex within primary cilia. INPP5E regulates phosphoinositide levels important for ciliary function and signaling impacting various downstream biological processes.
Pathways
PMPCA is integrated into the mitochondrial import pathway important for functional validation of mitochondrial metabolic and respiratory pathways. It interacts with proteins like HSP60 and HSP10. INPP5E influences the phosphoinositide-dependent signaling pathway vital for maintaining the stability and function of primary cilia. This pathway associates with proteins such as tubby-like protein 3 (TULP3) orchestrating ciliary membrane signaling events.
PMPCA relates to neurodegenerative conditions such as spinocerebellar ataxia due to its role in ensuring mitochondrial protein processing. Connections exist with proteins like mitochondrial DNA-directed RNA polymerase contributing to the disorder. INPP5E is linked to ciliopathies like Joubert syndrome because of abnormalities in ciliary phosphoinositide levels. The involvement of INPP5E in these disorders often relates to its interaction with the ARL13B protein which influences ciliary biology and pathogenesis.