Abcam, ab140640, Anti-HARS antibody [EPR9450(B)(ABC)]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal HARS antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR9450(B)(ABC),
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
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contact us
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Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HARS also known as Histidyl-tRNA synthetase is an enzyme involved in the translation process of protein synthesis. It charges tRNA molecules with histidine facilitating the incorporation of this amino acid into growing polypeptide chains. HARS has an approximate molecular mass of 57 kDa. The enzyme is widely expressed in human tissues including high levels in the liver and skeletal muscle which supports its role in the active protein synthesis associated with these organs.
Biological function summary
Histidyl-tRNA synthetase plays a fundamental role in ensuring accuracy during protein synthesis by attaching histidine to its corresponding tRNA. This enzyme acts as a monomer but often interacts with other synthetases in a multisynthetase complex. By providing histidine-charged tRNAs HARS supports the translation machinery that synthesizes proteins based on encoded genetic instructions reflecting its essentiality in cellular health and function.
Pathways
HARS operates predominantly within the aminoacyl-tRNA biosynthesis pathway which is essential for protein translation. This pathway involves several other aminoacyl-tRNA synthetases including the leucyl-tRNA synthetase (LARS) and lysyl-tRNA synthetase (KARS) which facilitate the attachment of specific amino acids to their respective tRNAs. Additionally HARS links to the broader mTOR signaling pathway a critical pathway for regulating cell growth and protein synthesis in response to nutrient availability.
HARS mutations have association with a range of pathologies particularly autoimmune conditions such as myositis. Changes in HARS expression or function are also observed in neurodegenerative diseases like Charcot-Marie-Tooth disease a disorder characterized by peripheral nerve damage. Connections to these diseases highlight interactions between HARS and proteins involved in immune response and nerve function although the precise mechanisms may require further study.