Abcam, ab140808, Recombinant Human SMAD9 protein

Size: 20µg
Recombinant Human SMAD9 protein is a Human Full Length protein, in the 1 to 467 aa range, expressed in Escherichia coli, with >70%, suitable for SDS-PAGE, WB.
Key facts
Purity:>70% Densitometry,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:SDS-PAGE, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:O15198,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SMAD9 also known as Mothers against decapentaplegic homolog 9 or MADH9 is a transcription factor involved in signal transduction and transcriptional activity. It has a molecular mass of approximately 50 kDa. SMAD9 is widely expressed in various tissues including the heart brain and skeletal muscle. In its mechanical function SMAD9 becomes activated through phosphorylation by type I receptor serine/threonine kinases and forms part of the SMAD protein family that transmits extracellular signals to the nucleus affecting gene expression.
Biological function summary
SMAD9 plays a role in the cellular processes of growth and differentiation particularly within the bone morphogenetic protein (BMP) signaling pathway. It operates as part of a complex with other SMAD proteins such as SMAD1 and SMAD5 forming heteromeric complexes that regulate the transcription of BMP-responsive genes. This gene transcription is vital for the development and repair of tissues as well as the maintenance of cellular homeostasis.
Pathways
SMAD9 participates significantly in the BMP and transforming growth factor-beta (TGF-β) signaling pathways. These pathways are important for a variety of cellular responses including proliferation apoptosis and differentiation. SMAD9 associates with BMP receptors to mediate the BMP signaling cascade acting synergistically with other SMAD family members like SMAD4 which is a common mediator in these pathways. This interrelation places SMAD9 at a pivotal point within the regulation of multiple cellular responses.
Research indicates that SMAD9 mutations or dysregulation can associate with pulmonary arterial hypertension (PAH) and certain forms of cancer. In PAH altered SMAD9 signaling disrupts normal vascular remodeling resulting in elevated pulmonary artery pressure. Additionally it can interact with other proteins like BMPR2 a receptor for BMP which also plays a significant role in the pathogenesis of PAH. Understanding the involvement of SMAD9 in these conditions can provide insights into potential therapeutic targets for treatment.