Abcam, ab14083, Propidium Iodide

Size: 1mL
Propidium Iodide (PI) ab14083 is provided in a convenient solution for staining double-stranded DNA. Propidium Iodide (PI) ab14083 is a fluorescent DNA stain that specifically binds to double-stranded DNA where the plasma membrane has been compromised/ permeabilized. It does not penetrate live cells. - Use in fluorescence microscopy and flow cytometry - Cited in over 50 publications.
Key facts
Applications:Flow Cyt, Fluorescence MicroscopySee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Form:LiquidSee storage information,
Storage buffer:pH: 7.4Constituents: 99.9% PBS, 0.1% Propidium Iodide

Product details:
Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized.
For Microscopy analysis
: PI can be viewed using rhodamine(red) filter (λ= 536/617). Cells will only be stained if the membrane has been permeated, either naturally (non-viable cells) or with detergents (for fluorescent staining).
For Flow Cytometry analysis:
PI staining can be monitored in FL2 channel.
If used together as control for Annexin V assays
ab14082
, ab14083 or
ab14152
, PI should be diluted to 250 μg/ml solution (in PBS) prior use and added as 1 μl/ Annexin V Assay (0.25μg/assay).
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for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called 1056 Propidium Iodide. 1056-1 is the same size as the 1 ml size of ab14083.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C