Abcam, ab14460, Anti-Poly (ADP-Ribose) Polymer antibody

Size: 100µg
Chicken Polyclonal Poly (ADP-Ribose) Polymer antibody. Suitable for ELISA, WB and reacts with Chemical samples. Cited in 19 publications.
Key facts
Host species:Chicken,
Clonality:Polyclonal,
Isotype:IgY,
Carrier free:No,
Applications:WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody recognizes Poly (ADP-Ribose) Polymer synthesized by a variety of poly(ADP-ribose) polymerases (PARP)-related enzymes, including PARP1, 2, 3, tankyrase, vPARP, sPARP and others. It does not cross-react with ADP-ribose, 5'-AMP, or yeast RNA as tested by ELISA. It does cross-react to bovine serum albumin due to its use as a carrier for the immunogen.

Properties and Storage Information:
Form-Liquid, Purity-IgY fraction, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: 0.87% Sodium chloride, 0.316% Tris HCl, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Poly (ADP-Ribose) Polymerase often abbreviated as PARP is an enzyme that plays an important role in cellular processes. PARP catalyzes the addition of ADP-ribose units from NAD+ to target proteins—a process known as poly(ADP-ribosyl)ation. This modification is important for modulating protein function. The enzyme has a molecular weight of approximately 116 kDa. PARP is located mainly in the nucleus of cells and shows high expression in tissues with elevated cellular proliferation and metabolism such as the brain and testis.
Biological function summary
PARP is integral to the DNA damage response mechanism in eukaryotic cells. It detects and signals single-strand DNA breaks inducing repair by recruiting DNA repair complexes. PARP collaborates with XRCC1 to facilitate the base excision repair pathway. As a part of these complexes PARP’s activity ensures cell survival under stress conditions. In concert with other poly (ADP-ribose) molecules PARP also influences chromatin structure impacting gene expression.
Pathways
PARP functions prominently within the signaling responses to DNA damage and apoptosis pathways. It associates closely with proteins like ATM and ATR as they coordinate cell cycle checkpoints. PARP’s enzymatic activity modulates the synthesis of poly (ADP-ribose) which interacts with other repair proteins to drive DNA strand repair. Furthermore the activity of PARP connects to the tumor suppressor protein p53 influencing cellular fate decisions between cell survival and apoptosis.
PARP is heavily studied in the context of cancer and neurodegenerative diseases. Elevated PARP activity is recognized in various cancers as it helps tumor cells survive DNA damage. PARP inhibitors have emerged as therapeutic agents targeting cancers with BRCA1/2 mutations. Moreover in neurodegenerative diseases like Parkinson’s PARP’s excessive activation can lead to cell death. The interaction of PARP with other proteins such as α-synuclein in Parkinson’s highlights its multifaceted role in disease pathogenesis.