Abcam, ab14730, Anti-ATPB antibody [3D5] - Mitochondrial Marker

Size: 100µg
Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730) is a mouse monoclonal antibody detecting ATPB in Western Blot, Flow Cytometry, IHC-P, ICC/IF . Suitable for Caenorhabditis elegans, Cow, Human, Mouse, Rat . - Over 250 publications - Trusted since 2004
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3D5,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Cow, Human, Caenorhabditis elegans,
Applications:Flow Cyt, IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:Human and Bovine complex V beta subunit (ATPB).

Product details:
What is this antibody validated in?
Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Caenorhabditis elegans, Cow, Human, Mouse, Rat samples.
What is the molecular weight of ATPB?
Anti-ATPB [3D5] - Mitochondrial Marker (ab14730) specifically detects a band for ATPB (UniProt: P06576) at a molecular weight of 52kDa.
Trusted by the scientific community
Anti-ATPB [3D5] - Mitochondrial Marker (ab14730) was first used in a scientific publication in 2004 and has been cited over 250 times in peer-reviewed journals.
Reviewed by scientists
Anti-ATPB [3D5] - Mitochondrial Marker (ab14730) has over 40 independent reviews from customers.
Other related products
We have a range of other formats of antibody clone [3D5] also available for your convenience: ab14730, Alexa Fluor® 647 -
ab197649
, Alexa Fluor® 488 -
ab197904
, HRP -
ab197905
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Purification notes-Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation., Storage buffer-pH: 7.4 - 7.5Preservative: 0.02% Sodium azideConstituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATPB also known as ATP synthase subunit beta is an essential protein component of the ATP synthase complex. It has an approximate mass of 52 kDa and is primarily expressed in the mitochondria. The protein's role is to catalyze the production of adenosine triphosphate (ATP) from adenosine diphosphate (ADP) and inorganic phosphate utilizing the proton gradient across the inner mitochondrial membrane. This process is central to the cellular energy production often referred to as oxidative phosphorylation. ATPB is frequently used as a mitochondrial marker in research making it an important target for antibodies such as those conjugated with Alexa Fluor 647 for immunofluorescence applications.
Biological function summary
ATPB functions as part of the mitochondrial ATP synthase complex which is also known as complex V of the electron transport chain. This complex is important for maintaining cellular energy homeostasis through ATP production. ATPB contributes to the catalytic activity necessary for ATP synthesis therefore supporting various cellular processes that require energy input such as muscle contraction and active transport. The protein also plays a role in coupling the proton motive force to ATP synthesis a function critical for mitochondrial efficiency and metabolic health.
Pathways
ATPB involves itself significantly in the oxidative phosphorylation and glycolysis pathways. It partners with other proteins in the ATP synthase complex such as ATP synthase subunit alpha (ATP5A1) to effectuate the conversion of energy. In the broader scope of energy metabolism ATPB integrates with glycolysis where glycolytic end-products feed into oxidative phosphorylation sustaining the cell’s energy currency. Both pathways are important for cells especially in tissues with high energy demands like the heart and skeletal muscles.
ATPB has been implicated in mitochondrial dysfunction-related diseases such as mitochondrial myopathy and Leigh syndrome. These conditions often result from mutations or defects in components of the electron transport chain leading to impaired ATP production. ATPB’s close connection to ATP5A1 and other complex V proteins highlights its involvement in these disorders. Understanding ATPB's role and function helps in disease mechanism elucidation and potentially offers targets for therapeutic interventions in mitochondrial-related diseases.