Abcam, ab14984, Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade

Size: 100µg
Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade (ab14984) is a mouse monoclonal antibody detecting KAT3B / p300 in Western Blot, IP, ChIP . Suitable for Human . - Over 80 publications - Trusted since 2004
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3G230 / NM-11,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:ChIP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Published data suggest that this antibody cross-reacts with both CBP and P300 (PMID: 8995708 and 9219037). This paper (PMID: 9219037) also maps the epitope of this antibody to 2071-2091 of CBP.

Product details:
What is this antibody validated in?
Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade (ab14984) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), ChIP in Human samples.
What is the molecular weight of KAT3B / p300?
Anti-KAT3B / p300 [3G230 / NM-11] - ChIP Grade (ab14984) specifically detects a band for KAT3B / p300 (UniProt: Q09472) at a molecular weight of 300kDa.
Trusted by the scientific community
Anti-KAT3B / p300 [3G230 / NM-11] - ChIP Grade (ab14984) was first used in a scientific publication in 2004 and has been cited over 80 times in peer-reviewed journals.
Reviewed by scientists
Anti-KAT3B / p300 [3G230 / NM-11] - ChIP Grade (ab14984) has over 10 independent reviews from customers.
Other related products
We have a range of other formats of antibody clone [3G230 / NM-11] also available for your convenience: ab14984, HRP -
ab200186
, Carrier free -
ab264432
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
KAT3B also known as p300 is a histone acetyltransferase with a molecular weight of approximately 300 kDa. Known for its ability to acetylate histone and non-histone proteins p300 modulates gene expression by altering chromatin structure. The protein is ubiquitously expressed across various tissues impacting numerous cellular processes by its mechanical functions of modifying chromatin.
Biological function summary
KAT3B/p300 influences multiple cellular activities like cell growth differentiation and apoptosis. It functions as a transcriptional coactivator and integrates various signaling pathways into the cell's gene expression program. p300 often forms complexes with other transcription factors enhancing or repressing their activity based on the cellular context. By modifying transcription factors it plays an essential role in controlling cell fate decisions.
Pathways
P300 participates in the regulation of significant cellular processes including the Wnt signaling pathway and the p53 pathway. In the Wnt signaling pathway p300 acts alongside beta-catenin to regulate gene expression while in the p53 pathway it acetylates the p53 protein influencing the cell’s response to DNA damage. These pathways highlight p300's interaction with important proteins such as CBP (CREB-binding protein) which shares a similar functional repertoire and works synergistically in gene regulation.
P300 is implicated in cancers and congenital disorders. In cancer aberrant regulation or mutation of p300 can lead to uncontrolled cell proliferation and tumor growth; p300 inhibitors like C646 are researched for their therapeutic potential. Additionally Rubinstein-Taybi syndrome a congenital disorder results from mutations in the p300 gene showcasing p300’s importance in developmental processes. The interplay of p300 with proteins such as CBP is also relevant in these conditions where disrupted interactions may lead to disease manifestation.