Abcam, ab150077, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Size: 500µg
Goat anti-rabbit IgG H&L (Alexa Fluor® 488) is a secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA. - Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets - Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis - Cited in over 3100 publications
Key facts
Host species:Goat,
Target species:Rabbit,
Target isotype:IgG,
Target specificity:Heavy & Light chains,
Pre-adsorbed:No,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Applications:IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Clonality:Polyclonal,
Isotype:IgG,
Specificity:This antibody is specific to Rabbit IgG.

Product details:
Fluorochrome chart
- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers
a comprehensive suite of IHC kits and secondary antibodies
, designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-This antibody was isolated by affinity chromatography using antigen coupled to agarose beads., Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Immunoglobulin G (IgG) serves as an essential component of the immune system functioning mainly as an antibody. It is found in blood and extracellular fluid making it one of the most prevalent antibodies in the human body. IgG weighs approximately 150 kDa and it is expressed by B cells as part of the humoral response to pathogens. Alternate names include gamma globulin and Ig gamma. IgG antibodies form a critical aspect of adaptive immunity by targeting specific antigens for neutralization or destruction.
Biological function summary
IgG participates in several immune processes by binding to antigens and forming immune complexes. It facilitates pathogen opsonization initiating phagocytosis by immune cells like macrophages and neutrophils. IgG also activates the classical complement pathway which enhances pathogen clearance. IgG exists in a monomeric form and can cross the placenta providing passive immunity to the fetus. In laboratory settings its high specificity makes it valuable in techniques like IgG ELISA for detecting antigen presence.
Pathways
IgG plays an integral role in the complement system and the adaptive immune response. It partners with proteins such as C1q to initiate the classical complement pathway amplifying the body's ability to detect and remove pathogens. IgG also interacts with Fc receptors on immune cells modulating immune cell activity and facilitating antigen presentation to T cells. These interactions enhance the immune system's efficiency in recognizing and responding to diverse pathogens.
IgG has connections to autoimmune diseases and chronic infections. Elevated levels of IgG can indicate autoimmune conditions like lupus or rheumatoid arthritis where the immune system mistakenly attacks healthy tissue. During chronic infections such as HIV variations in IgG responses can affect disease progression and treatment efficacy. In these contexts IgG interacts with proteins involved in the immune dysregulation characteristic of such diseases highlighting its significant role in both protection and pathology.