Abcam, ab150662, Alcian Blue Stain Kit (pH 2.5, Mucin Stain)

Size: 30mL / 250mL
Alcian Blue Stain Kit (pH 2.5, Mucin Stain) ab150662 is used to stain tissue sections for sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins). - Suitable for formalin-fixed paraffin-embedded sections - Cited in over 30 publications
Product details:
Alcian Blue Stain Kit (pH 2.5, Mucin Stain) ab150662 is intended for use in the histological visualization of sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins).
This Kit originally used Safranin O Solution but this has now been replaced with Nuclear Fast Red Solution.
Alcian Blue staining protocol summary:
- deparaffinize sections if necessary and hydrate
- incubate in acetic acid for 3 min
- incubate in Alcian Blue pH 2.5 for 30 min
- rinse in water
- stain in nuclear fast red for 5 min
- rinse in water
- dehydrate, clear and mount
Other products for staining tissue sections
Find more kits and reagents in the
special stains guide
, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the
IHC kits and reagents guide
Staining Interpretation
Acidic Sulfated Mucosubstances
Hyaluronic Acid
Sialomucins
Nuclei
Background
Control Tissue:
Small Intestine, Appendix, Colon
Previously called Alcian Blue pH 2.5 Stain Kit (Mucin Stain)
Other Notes
Please note, the 250 mL selling size, was previously described as 100 Test. There has been no change to the physical product.

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient, Storage information-Ambient