Abcam, ab153274, Recombinant Human Tin2 protein

Size: 10µg
Recombinant Human Tin2 protein is a Human Fragment protein, in the 256 to 354 aa range, expressed in Wheat germ, suitable for ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9BSI4,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TIN2 also known as TERF1-interacting nuclear factor 2 is a pivotal protein involved in telomere maintenance. It has a molecular mass of approximately 50 kDa. This protein is expressed in the nucleus where it plays an integral role in structuring chromosome ends. TIN2 specifically associates with telomeric regions to regulate their length and integrity. It forms connections with other proteins to facilitate its function in telomers' structural configuration.
Biological function summary
TIN2 integrates into the shelterin complex a group of six proteins that protect telomeres from being recognized as DNA damage. It acts as a stabilizer within this complex and helps maintain telomere integrity. In doing so TIN2 modulates the actions of telomerase and other telomere-binding proteins to ensure proper chromosome end function. This responsibility makes it essential for preventing excessive telomere shortening which can lead to genomic instability.
Pathways
The involvement of TIN2 is significant in telomere length regulation and DNA damage response mechanisms. TIN2 operates within these pathways to manage telomerase activity and interaction with telomere ends. It collaborates with other shelterin proteins like TRF1 and TRF2 which also participate in maintaining telomere function. TIN2's role in these pathways highlights its contribution to chromosome end protection and preventive response against DNA damage.
TIN2 dysfunction is linked to conditions like dyskeratosis congenita and aplastic anemia. These disorders are marked by defective telomere maintenance leading to progressive bone marrow failure and other cellular deficiencies. Mutations in TIN2 can perturb the function of shelterin proteins including TRF1 contributing to the compromised telomere integrity seen in these diseases. Understanding TIN2's role offers insights into therapeutic targets for managing telomere-associated disorders.