Abcam, ab153803, Anti-FBXO4/FBX4 antibody

Size: 100µL
Rabbit Polyclonal FBXO4/FBX4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FBXO4 aa 1-350.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human FBXO4 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.Q9UKT5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FBXO4 also called F-box-only protein 4 or FBX4 acts as an E3 ubiquitin ligase component within the SCF complex. It facilitates the ubiquitination and proteasomal degradation of certain proteins by recognizing phosphorylated substrates. FBXO4's molecular mass is around 45 kDa. This protein expresses across various tissues including the brain and heart and also plays a role in the cell cycle regulation and signal transduction.
Biological function summary
FBXO4 is involved in controlling the stability of important regulatory proteins. It assembles with SKP1 Cul1 and RBX1 to form the SCF complex. One of its known substrates is cyclin D1 which is important in regulating cell cycle progression. Degrading cyclin D1 helps prevent the abnormal cell growth associated with cancer development.
Pathways
FBXO4 functionally integrates into the ubiquitin-proteasome system and cell cycle pathways. In the ubiquitin-proteasome pathway it works alongside other enzymes to ensure targeted protein degradation important for maintaining cellular homeostasis. Inside the cell cycle pathway FBXO4's interaction with cyclin D1 influences transition phases coordinating with other proteins like CDK4 to regulate cell division and growth.
FBXO4 has associations with certain cancers like melanoma and breast cancer. Dysregulation of FBXO4 leading to impaired degradation of cyclin D1 contributes to tumorigenesis and cancer progression. Interaction with proteins such as SKP2 involved in cell cycle regulation and cancer highlights the pathogenic potential of altered FBXO4 function when it impacts cell growth control.