Abcam, ab154027, Anti-GLUD1 + GLUD2 antibody

Size: 100µL
Rabbit Polyclonal GLUD1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GLUD2 aa 250 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human, Rat,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human GLUD2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P49448

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GLUD1 and GLUD2 also known as glutamate dehydrogenase 1 and 2 are enzymes that catalyze the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. The mass of GLUD1 is approximately 61 kDa and it is expressed in multiple tissues with high levels in the liver brain and pancreas. GLUD2 is expressed mainly in the brain. These enzymes are integral for regulating amino acid metabolism and are activated by ADP and inhibited by GTP.
Biological function summary
GLUD1 and GLUD2 influence cellular energy homeostasis by modulating levels of glutamate and alpha-ketoglutarate. They function as homodimers or heterodimers and play a role in neurotransmitter cycling in neural tissues. This involvement in neurotransmitter metabolism is significant as they ensure glutamate levels remain balanced preventing excitotoxicity.
Pathways
GLUD1 and GLUD2 participate in the urea cycle and glutamate metabolism. They work alongside other enzymes like glutamine synthetase and aspartate aminotransferase to maintain nitrogen and energy balance in cells. Glutamine synthetase helps convert the ammonia released into glutamine which is vital for cellular function.
GLUD1 is linked to hyperinsulinism/hyperammonemia syndrome caused by mutations that lead to dysregulated insulin secretion. Disruptions in GLUD2 activity have implications in neurological disorders such as schizophrenia where it affects glutamate signaling. Both enzymes interact with insulin pathways and can influence disease states through these interactions.