Abcam, ab154343, Anti-MAT2A antibody

Size: 100µL
Rabbit Polyclonal MAT2A antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAT2A aa 1-300.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human MAT2A aa 1-300. The exact immunogen used to generate this antibody is proprietary information.P31153

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MAT2A or methionine adenosyltransferase 2A is an enzyme that plays an important role in the methionine cycle by catalyzing the conversion of methionine to S-adenosylmethionine (SAM). It has a molecular mass of approximately 43 kDa. MAT2A is widely expressed in various tissues with high levels typically found in the liver and kidney. This protein has alternative names including MAT-II and AdoMet synthetase. Its activity is critical for maintaining cellular methylation processes that affect a vast array of cellular functions.
Biological function summary
Methionine adenosyltransferase 2A is essential for the synthesis of SAM which is a primary methyl donor in cellular methylation reactions. MAT2A often works as part of a larger protein complex that regulates cellular growth and division. It is not an isolated component but participates in complexes that ensure proper methyl donation throughout various biological activities. The enzyme's role in methylation impacts DNA RNA and protein modifications which are essential for gene expression regulation and cellular response.
Pathways
Methionine adenosyltransferase 2A is significant in the methionine metabolism pathway closely interlinking with the transsulfuration pathway. This pathway involvement connects MAT2A with key metabolic regulators like SAM and cystathionine beta-synthase ensuring balanced methionine homeostasis and redox status. MAT2A also interacts with proteins like MAT1A which performs similar functions in the liver showing interdependency within pathways that govern methylation and sulfur amino acid metabolism.
Dysregulation of the methionine adenosyltransferase 2A has been implicated in liver cancer and neurodegenerative diseases. Aberrant expression or mutations in MAT2A can lead to disrupted SAM synthesis which contributes to altered methylation patterns observed in hepatocellular carcinoma (HCC). Additionally changes in MAT2A expression are relevant to neurodegenerative conditions as it interacts indirectly with proteins such as MAT1A which is also involved in hepatic pathologies. Understanding MAT2A's role provides insights into potential therapeutic targets for these conditions.