Abcam, ab155454, Mouse Decorin ELISA Kit (DCN)

Size: 1 x 96Tests
Mouse Decorin ELISA Kit (DCN) is a Sandwich (quantitative) ELISA for the measurement of Mouse Decorin (DCN) in Mouse in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:< 5 pg/mL,
Range:4.1 - 1000 pg/mL,
Assay time:5h,
Assay Platform:Microplate

Product details:
Abcam's Decorin (DCN) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse Decorin in serum, plasma (collect plasma using EDTA and heparin as an anticoagulant. Citrate is not recommended as anticoagulants for use in this assay).
This assay employs an antibody specific for mouse Decorin coated on a 96-well plate. Standards and samples are pipetted into the wells and Decorin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Decorin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Decorin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get better reproducibility in only 90 minutes with Mouse Decorin ELISA Kit (
ab207618
) from our SimpleStep ELISA
range.
Optimization may be required with urine samples.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C