Abcam, ab155458, Mouse MPO ELISA Kit (Myeloperoxidase)

Size: 1 x 96Tests
Mouse MPO ELISA Kit (Myeloperoxidase) is a sandwich ELISA designed to quantify Mouse MPO (Myeloperoxidase) with a sensitivity of 0.6 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 0.61 - 150 ng/mL - Cited in over 20 publications
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:<= 0.61 ng/mL,
Range:0.61 - 150 ng/mL,
Assay Platform:Microplate

Product details:
Mouse MPO ELISA Kit (Myeloperoxidase) ab155458 is a sandwich ELISA to measure Mouse MPO (Myeloperoxidase) in plasma, cell culture supernatant, cell lysate with a sensitivity of 0.6 ng/ml.
How the assay works
This assay employs an antibody specific for mouse MPO coated on a 96-well plate. Standards and samples are pipetted into the wells and MPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse MPO ELISA Kit (Myeloperoxidase) ab155458 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.
Biological function summary
The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.
Pathways
Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.
Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.