Abcam, ab155891, Total Carbohydrate Assay Kit - Quantification

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Total Carbohydrate Assay (ab155891) is a simple, sensitive and robust method of detecting virtually all carbohydrates.
Key facts
Detection method:Colorimetric,
Sample types:Suspension cells, Tissue Extracts, Adherent cells, Food samples, Drinks,
Assay type:Quantitative,
Assay time:30m,
Assay Platform:Microplate reader

Product details:
Total Carbohydrate Assay (ab155891) is a simple, sensitive and robust method of detecting virtually all carbohydrates.
How the assay works
The carbohydrate assay protocol is based on the phenol - sulfuric acid assay. In the assay, polysaccharides (mono, di, tri, etc.) and their derivatives, in the presence of sulfuric acid, are hydrolyzed to monomers and converted to furfural or hydroxyfurfural. These react with the developer to form a chromogen that can be quantified by measuring the absorbance at 490 nm.
Total carbohydrate assay protocol summary:
- Add samples and glucose standards to wells
- Add concentrated sulfuric acid and incubate for 15 min at 90°C
- Add developer and incubate for 5 min
How other researchers are using ab155891
Total Carbohydrate Assay Kit
ab155901
has been used in a variety of sample type including:
SHK-1 cell line
Blood samples from mice with metabolic syndrome
Lattuce seeds extracts
References: 1-Martinez D et al. 2022, 2-Greco C et al. 2020, 3-Rawashdeh Y et al. 2020
Related and recommended products
See an alternative kit to quantify carbohydrates:
High Sensitivity Carbohydrate Assay Kit (Colorimetric)
ab284543
Other Notes
This product was previously called K645 Biovision Total Carbohydrate Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.
Carbohydrates play important structural as well as chemical roles in all living systems. Detection of total carbohydrates, therefore, has wide applications.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Please refer to protocols