Abcam, ab155895, Peroxidase Activity Assay Kit

Size: 100Test
Peroxidase Assay Kit ab155895 provides a convenient colorimetric and fluorometric means to measure peroxidase activity in biological samples. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Urine, Plasma, Cell culture supernatant, Serum, Other biological fluids,
Assay type:Enzyme activity,
Assay Platform:Microplate reader

Product details:
Peroxidase Assay Kit ab155895 provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples. In the presence of Peroxidase, the OxiRed Probe reacts with H
in a 1:1 stoichiometry to produce the red fluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (570nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, direct, highly sensitive and high throughput-ready. The detection limit is 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.
Other Notes
This product was previously called K772 Peroxidase Activity Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.
Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR' + electron donor (2 e-) + 2H+ to ROH + R'OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C