Abcam, ab157108, Anti-hUPF2/RENT2 antibody

Size: 100µg
Rabbit Polyclonal hUPF2/RENT2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human UPF2 aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human UPF2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.Q9HAU5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab157108 was affinity purified using an epitope specific to hUPF2/RENT2 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The human UPF2 protein also known as RENT2 is a significant component in the mechanism of nonsense-mediated mRNA decay (NMD). This protein weighs approximately 140 kDa and is an important factor in ensuring quality control within mRNA processes. It is expressed widely in various human tissues playing a central role in cellular regulation processes. UPF2 is recognized for its dual role as a scaffold protein and a bridging factor facilitating interactions between other key components of the NMD pathway demonstrating how essential it is for proper mRNA surveillance.
Biological function summary
UPF2 acts as a central component of the NMD complex a critical surveillance system that degrades mRNAs containing premature stop codons. It recruits and interacts with other essential NMD factors like UPF1 and UPF3 forming a complex that ensures correct mRNA splicing and translation. By binding to these proteins UPF2 stabilizes the assembly of the NMD complex playing an important role in recognizing defective mRNAs and initiating their decay. Its efficient operation keeps potentially harmful or non-functional proteins from accumulating within the cell.
Pathways
UPF2 is deeply involved in the nonsense-mediated mRNA decay pathway. This pathway acts as a cellular defense mechanism against aberrant mRNA transcripts. The UPF2 protein collaborates closely with UPF1 an ATP-dependent helicase that triggers the decay process when it detects faulty mRNA. Additionally UPF2 links with other proteins like SMG-1 kinase which phosphorylates UPF1 leading to the activation of NMD. As such UPF2's function within these pathways is integral to maintaining cellular integrity by regulating gene expression.
Dysfunction in UPF2-related activities contributes significantly to certain genetic disorders and cancers. Mutations or malfunctions in the NMD pathway where UPF2 is a major player have been linked to neurological disorders such as some types of intellectual disabilities. In cancers arising defects in NMD can cause the accumulation of oncogenic proteins promoting tumorigenesis. Additionally UPF2 interactions with SMG-1 and UPF1 proteins are critical in managing these processes highlighting its relevance to disease pathogenesis and potential therapeutic interest.