Abcam, ab157117, Anti-SF3B4 antibody

Size: 100µg
Rabbit Polyclonal SF3B4 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human SF3B4 aa 350 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SF3B4 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q15427

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SF3B4 also known as SAP49 is a component of the spliceosome complex involved in pre-mRNA splicing. This protein weighs around 49 kDa and is expressed in various human tissues with especially high levels in the liver kidney and brain. SF3B4 functions by binding to pre-mRNA substrates and plays an essential role in recognizing splice sites an important step in the splicing process. SF3B4 along with other SF3B family members forms part of the U2 small nuclear ribonucleoprotein (snRNP) complex contributing to the accurate selection and removal of introns from pre-mRNA.
Biological function summary
SF3B4 participates in the precise editing and maturation of pre-mRNA during gene expression. As part of the U2 snRNP complex it collaborates with other factors in the assembly of the spliceosome. This complex ensures the precursor mRNA is properly spliced to produce mature mRNA closely linking SF3B4’s activity to efficient genetic information processing. SF3B4's function is especially important in ensuring the fidelity of the splicing mechanism which affects both mRNA stability and translational efficiency.
Pathways
The activity of SF3B4 is importantly tied to the RNA splicing and mRNA processing pathways. It interacts with other critical proteins like SF3B1 and SF3B2 which together facilitate the correct assembly and function of the spliceosome. This involvement in splicing pathways affects gene expression regulation an essential component of cellular differentiation and development. Disruption in SF3B4 or its associated proteins can lead to defective mRNA splicing impacting multiple downstream biological processes.
SF3B4’s function correlates with several splicing-related pathologies such as Nager syndrome and various cancers. Nager syndrome a rare congenital disorder involves mutations in SF3B4 that disrupt normal splicing processes. Additionally expression changes in SF3B4 have connections with oncogenesis and its interaction with other SF3B proteins contributes to aberrant splicing patterns often observed in cancerous cells. These interactions highlight its potential as a therapeutic target in disease treatment and management.