Abcam, ab157464, Anti-MBD3 antibody [EPR9913] - ChIP Grade

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal MBD3 antibody. Suitable for IP, ChIP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human, Cow samples. Cited in 24 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR9913,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human, Rat, Cow,
Applications:Flow Cyt (Intra), IP, ICC/IF, WB, ChIPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The MBD3 protein also known as Methyl-CpG-binding domain protein 3 functions mechanically by binding to methylated DNA which is an essential step in regulating gene expression. It has a molecular mass of approximately 32 kDa. MBD3 is ubiquitously expressed in various tissues demonstrating its role in fundamental cellular processes. The protein does not function alone and often interacts with other proteins as part of gene repression mechanisms.
Biological function summary
The activity of MBD3 impacts chromatin remodeling and gene expression regulation. MBD3 operates as a core component of the nucleosome remodeling deacetylase (NuRD) complex a multi-protein assembly important for transcriptional repression and chromatin structure modification. Its interaction within this complex helps modulate gene expression by altering the accessibility of chromatin and recruiting other modifiers for further regulation.
Pathways
MBD3 plays a significant role in key signaling and epigenetic pathways. It engages in pathways related to chromatin modification and transcription regulation. Its role in the NuRD complex underlines its participation in transcriptional regulation pathways impacting proteins such as HDAC1 and GATA2 which contribute to modifying histone acetylation and influencing gene transcription processes.
Changes in MBD3 expression or function have links to cancer and neurodevelopmental disorders. MBD3 dysregulation associates with tumorigenesis due to its role in maintaining proper gene silencing and expression. Additionally impairment in MBD3 function can relate to intellectual disabilities and developmental delays potentially due to its interaction with epigenetic regulators like MeCP2.