Abcam, ab157708, Human IgD ELISA Kit

Size: 1 x 96Tests
Human IgD ELISA Kit is a sandwich ELISA designed to quantify Human IgD with a sensitivity of 4.4545 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 6.25 - 400 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum, Other biological fluids,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 4.4545 ng/mL,
Range:6.25 - 400 ng/mL,
Assay Platform:Microplate

Product details:
Human IgD ELISA Kit ab157708 is a sandwich ELISA to measure Human IgD in serum, plasma, other biological fluids with a sensitivity of 4.4545 ng/ml.
How the assay works
In this assay the IgD present in samples reacts with the anti-IgD antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgD antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgD. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgD in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgD in the test sample. The quantity of IgD in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human IgD ELISA Kit ab157708 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well.
6. Immediately begin recording the color development

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-Multi, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IgD also known as Immunoglobulin D serves as a distinct antibody isotype expressed primarily on the surface of B cells. It is involved in the initiation of immune responses. IgD has a molecular weight of approximately 180 kDa. The full form of IgD is Immunoglobulin D. It is expressed mainly in the respiratory system the tonsils and lymphoid tissues playing a pivotal role in the early stages of immunity. Researchers often perform IgD tests to study its mechanical aspects and influence on immune cell functionality.
Biological function summary
IgD contributes to the activation and differentiation of B lymphocytes and may influence antibody affinity maturation. This immunoglobulin is not known to form part of a larger complex but acts in conjunction with other immunoglobulins like IgM to regulate immune responses. The presence of IgD on B cells suggests its involvement in signaling processes which help B cells transition from immature to fully functional states impacting how the immune system responds to pathogens.
Pathways
IgD plays roles in immune system signaling and pathogen recognition. It appears in pathways such as B cell receptor signaling and participates in complex interactions with other proteins such as IgM and CD79 which are essential for transmitting activation signals. These pathways are important enabling the immune system to identify and respond to antigens effectively offering a first line of defense against infections.
IgD has associations with recurrent respiratory infections and IgD myeloma a rare form of plasma cell neoplasm. The malfunction or dysregulation of IgD can impact B cell development and lead to immune system disorders. In such cases IgD levels often measured through IgD tests may serve as important indicators for monitoring disease progression. Moreover elevated levels of IgD can coexist with proteins such as IgE particularly in allergy-related responses and play roles in certain immunodeficiency conditions.