Abcam, ab157713, Mouse Ferritin ELISA Kit (FTL)

Size: 1 x 96Tests
Mouse Ferritin ELISA Kit (FTL) is a sandwich ELISA designed to quantify Mouse Ferritin (FTL) with a sensitivity of 3.1631 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 12.5 - 400 ng/mL - Cited in over 40 publications
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum, Other biological fluids,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 3.1631 ng/mL,
Range:12.5 - 400 ng/mL,
Assay Platform:Microplate

Product details:
Mouse Ferritin ELISA Kit (FTL) ab157713 is a sandwich ELISA to measure Mouse Ferritin (FTL) in serum, plasma, other biological fluids with a sensitivity of 3.1631 ng/ml.
How the assay works
In this assay the Ferritin present in samples reacts with the anti-Ferritin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Ferritin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ferritin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ferritin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ferritin in the test sample. The quantity of Ferritin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse Ferritin ELISA Kit (FTL) ab157713 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature.
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well.
5. Immediately begin recording the color development.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C