Abcam, ab157732, Rat Ferritin ELISA Kit

Size: 1 x 96Tests
Rat Ferritin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat Ferritin in Rat in Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum, Other biological fluids,
Reacts with:Rat,
Assay type:Sandwich (quantitative),
Sensitivity:= 2.7695 ng/mL,
Range:12.5 - 400 ng/mL,
Assay Platform:Microplate

Product details:
Abcam's Ferritin Rat ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative measurement of ferritin in rat biological samples.
In this assay the ferritin present in samples reacts with the anti-Ferritin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Ferritin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ferritin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ferritin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ferritin in the test sample. The quantity of Ferritin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols