Abcam, ab157742, Rat Kininogen ELISA Kit (KNG) ELISA Kit

Size: 1 x 96Tests
Rat Kininogen ELISA Kit (KNG) ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat Kininogen (KNG) in Rat in Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum,
Reacts with:Rat,
Assay type:Sandwich (quantitative),
Sensitivity:= 4.9707 ng/mL,
Range:6.25 - 400 ng/mL,
Assay Platform:Microplate

Product details:
Abcam's Kininogen (KNG) Rat ELISA Kit is a two-site enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Kininogen levels in rat serum and plasma.
In this assay the Kininogen present in samples reacts with the anti-Kininogen antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Kininogen antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Kininogen. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Kininogen in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Kininogen in the test sample. The quantity of Kininogen in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The kininogen is a protein involved in various biological processes and also known as high molecular weight kininogen (HMWK). This protein which has a mass of approximately 120 kDa circulates in the plasma. Kininogen is synthesized by the liver and endothelial cells where it exists as HMWK. It serves as a precursor to kinins like bradykinin which are important in regulating blood pressure and inflammation.
Biological function summary
Kininogen participates in the kallikrein-kinin system. It plays an important role in blood coagulation and the inflammatory response. HMWK is not part of a complex but interacts with factors such as prekallikrein and Factor XII. By serving as a co-factor HMWK facilitates the activation of these molecules which progresses the coagulation cascade and inflammatory pathways.
Pathways
The kininogen functions within the kallikrein-kinin and coagulation systems. It interacts closely with enzymes like kallikrein which cleaves kininogen to release bradykinin assisting in vasodilation and increasing vascular permeability. Additionally HMWK is tethered to the intrinsic pathway of coagulation by activation of Factor XII which links it to proteins involved in blood clot formation.
The kininogen is associated with hereditary angioedema and bradykinin-mediated edema. Abnormalities in its function can lead to excessive bradykinin release. This condition involves excessive swelling due to alterations in vascular permeability. Moreover the protein is linked to chronic inflammatory diseases through its connection with the bradykinin receptor and related peptides.