Abcam, ab160295, Recombinant Human CH25H protein

Size: 10µg
Recombinant Human CH25H protein is a Human Full Length protein, in the 1 to 272 aa range, expressed in Wheat germ, suitable for ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:O95992,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The CH25H protein also known as cholesterol 25-hydroxylase catalyzes the conversion of cholesterol into 25-hydroxycholesterol. This enzyme has a mass of approximately 33 kDa. It expresses widely in tissues but its level of expression is highest in macrophages and the liver. The protein is part of the enzymatic group that modulates lipid homeostasis and is integral to host defense mechanisms.
Biological function summary
Cholesterol 25-hydroxylase plays a significant role in lipid metabolism and immune regulation. It produces 25-hydroxycholesterol a potent oxysterol which exerts effects on lipid metabolic processes and immune cell signaling. While not part of a large multiprotein complex CH25H's activity impacts cellular cholesterol levels and potentially influences the production of certain signaling molecules.
Pathways
Cholesterol 25-hydroxylase contributes to the regulation of lipid metabolic pathways and innate immune responses. Within the lipid metabolism pathway CH25H interacts with proteins like LXR (liver X receptor) affecting cholesterol homeostasis. The enzyme also influences immune regulation by altering the production of signaling molecules that modulate the interferon response affecting pathways related to inflammation and innate immunity.
Cholesterol 25-hydroxylase has connections to atherosclerosis and viral infections. In the context of atherosclerosis its function in regulating cholesterol levels can influence plaque formation in arteries. The protein's involvement in immune response modulation links it to the body's defense against viral infections. Additionally CH25H's activity interacts with proteins involved in the immune response such as IFN-induced proteins to mount an antiviral state.