Abcam, ab16056, Anti-COX IV antibody - Mitochondrial Loading Control

Size: 100µg / 250µg
Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) is a rabbit polyclonal antibody detecting COX IV in Western Blot, IHC-P, IHC-Fr, ICC/IF . Suitable for Human, Mouse, Rat . - Over 270 publications - Trusted since 2005
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-Fr, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Product Specifications
Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-Fr, IHC-P, WB in african green monkey, chinese hamster, human, mouse, potato, rat, xenopus laevis samples.
COX IV antibodies are often used as loading controls in Western Blot. Anti-COX IV antibody - Mitochondrial Loading Control has been verified in Western Blot samples and detects a band at 17kDa Molecular weight.
Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) specifically detects COX IV (UniProt ID: P13073; Molecular weight: 17kDa) and is sold in 100 µg and 250 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) has high sensitivity and specificity.
Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) has been cited over 272 times in peer reviewed journals and is trusted by the scientific community.
Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) has 32 independent reviews from customers.
Target Information
COX IV, also known as cytochrome c oxidase, is a critical mitochondrial enzyme involved in oxidative phosphorylation and the respiratory chain. The COX IV molecular weight is approximately 17 kDa. Dysfunction in COX IV is linked to mitochondrial disorders and neurodegenerative diseases, highlighting its disease relevance. Understanding COX IV's role in key pathways, such as electron transport and ATP synthesis, is essential for developing targeted therapies for these conditions.
This antibody makes an effective loading control for mitochondria. COX IV is generally expressed at a consistent high level. However, be aware that many proteins run at the same 16kD size as COX IV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for proteins of this size. Some caution is required when using this antibody as a loading control as COXIV expression can vary under some manipulations. An alternative mitochondrial loading control is Mouse monoclonal to COX IV antibody [20E8] (
ab14744

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cytochrome c oxidase subunit IV commonly known as COX IV is a component of the enzyme complex located in the inner mitochondrial membrane. COX IV has a molecular weight of approximately 17 kDa and serves as a subunit of the larger cytochrome c oxidase complex which is essential in cellular respiration. As a mitochondrial marker COX IV is expressed in various tissues where it acts as an important player in the electron transport chain. The presence and function of COX IV are critical in facilitating the last step of the mitochondrial respiratory chain.
Biological function summary
COX IV acts as a significant part of the cytochrome c oxidase complex helping catalyze the reduction of oxygen to water. This process is an important step in the overall mechanism of oxidative phosphorylation. COX IV's role in this complex enables the proton gradient generation across the inner mitochondrial membrane which is necessary for ATP synthesis. Its activity regulates the efficiency of cellular respiration impacting energy production and metabolic activities within cells.
Pathways
COX IV functionally interacts within the oxidative phosphorylation and electron transport chain pathways. Its coordination with other proteins like COX I and COX II in the cytochrome c oxidase complex ensures proper electron transfer to oxygen. Additionally COX IV is implicated in the regulation of reactive oxygen species maintaining cellular homeostasis. These pathways interconnect with broader cellular mechanisms that involve energy metabolism and apoptosis.
COX IV has been linked to mitochondrial disorders where defects in the oxidative phosphorylation processes can lead to conditions such as mitochondrial encephalomyopathy. Abnormalities in COX IV function and expression can also contribute to neurodegenerative diseases including Parkinson's disease. Here interactions with proteins like superoxide dismutase (SOD) highlight how oxidative stress and mitochondrial dysfunction relate closely to disease progression. These associations underline the importance of COX IV in maintaining cellular and organismal health.