Abcam, ab162124, Recombinant Human RNF115 protein

Size: 10µg
Recombinant Human RNF115 protein is a Human Full Length protein, in the 1 to 304 aa range, expressed in Wheat germ, suitable for ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q9Y4L5,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Product details:
This product was previously labelled as ZNF364.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RNF115 also known as RING Finger Protein 115 or BRE1B is an E3 ubiquitin-protein ligase. This protein weighs approximately 34 kDa and belongs to the RBR (RING1-IBR-RING2) E3 ubiquitin ligase family. RNF115 functions mechanically by attaching ubiquitin molecules to specific protein substrates marking them for proteasomal degradation. RNF115 is found in various tissues showing higher expression in the brain and reproductive organs. It plays a mechanical role in proteostasis by facilitating the transfer of ubiquitin from E2 ubiquitin-conjugating enzymes to target proteins.
Biological function summary
RNF115 affects protein regulation through its involvement in ubiquitin-mediated processes. It interacts with different substrates and has been identified as part of the BRCA1-A complex which is important in DNA repair mechanisms. It helps regulate cellular levels and stability of proteins impacting cell cycle progression and response to DNA damage. Its activity ensures proper cellular development and homeostasis by maintaining protein turnover.
Pathways
RNF115 engages in the ubiquitin-proteasome system and is implicated in the DNA damage response pathways. It acts within the ubiquitination pathways collaborating with other proteins like BRCA1 and UBE2D1 to influence protein degradation and DNA repair processes. RNF115's role in the regulation of the DNA damage response highlights its connection to cellular stress resilience linking it to cellular pathways that maintain genetic stability.
Alterations in RNF115 expression or function can link to cancer development and neurodegenerative diseases. Misregulation can impact pathways involving BRCA1 associated with breast cancer contributing to abnormal cell proliferation and genomic instability. Additionally disruptions in its protein degradation function may relate to neurodegenerative diseases where abnormal protein accumulation occurs such as Alzheimer's disease. The connection of RNF115 with proteins like BRCA1 and UBE2D1 in these conditions highlights its importance in maintaining cellular health and preventing disease progression.