Abcam, ab16388, Biotin Anti-Phosphotyrosine antibody [PY20]

Size: 100µg
Mouse Monoclonal Phosphotyrosine antibody - conjugated to Biotin. Suitable for ICC, IP, WB and reacts with Modified Amino Acid samples. Cited in 1 publication. Immunogen corresponding to Chemical / Small Molecule corresponding to Phosphotyrosine.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:PY20,
Isotype:IgG2b,
Conjugation:Biotin,
Carrier free:No,
Applications:IP, ICC, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:Clone PY20 specifically recognizes phosphorylated tyrosine residues and does not react with phosphorylated threonine or serine residues.

Product details:
The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine-phosphorylated proteins biochemical pathways in which they function.
This antibody is known to be inhibited by divalent cations (>1mM) and high salt concentrations (>0.2M).
The purified antibody is coupled through free primary amino groups to biotin. The binding site of the antibody was protected by the addition of phenyl phosphate prior to coupling.
Source: Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Storage buffer-pH: 7.5Preservative: 0.08% Sodium azideConstituents: PBS, 65% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Phosphotyrosine also known simply as pY is a post-translational modification where a phosphate group binds to the hydroxyl group of a tyrosine amino acid in proteins. This small modification can have a huge impact on proteins' function and interaction. Phosphotyrosine has a molecular weight of approximately 216 Da when it stands on its own. This modification can occur in various proteins expressed widely across tissues playing a role in signaling cell division and morphological changes.
Biological function summary
Phosphorylated tyrosine residues modify the function and activity of proteins within cells. It is a critical component of signal transduction pathways and can alter protein functions when becoming part of large protein complexes. It often regulates receptor proteins and intracellular kinases modifying their ability to interact with other proteins like SH2 and PTB domain-containing proteins. Phosphotyrosine antibodies like anti-phosphotyrosine Ig2 are helpful research tools for detecting these modifications.
Pathways
The modification of tyrosine to phosphotyrosine participates significantly in pathways such as the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways are important for various cellular processes including growth and survival. Proteins like EGFR and PDGFR regulate signaling cascades by phosphorylation of tyrosine residues and often interact with phosphotyrosine and phosphotyrosine antibodies importantly.
The dysregulation of phosphotyrosine levels contributes to conditions like cancer and diabetes. Aberrant activation or overexpression of phosphotyrosine residues on receptors like HER2 and insulin receptors can promote uncontrolled cell proliferation often seen in tumors and contribute to insulin resistance respectively. As such phosphotyrosine serves not only as a biological target but also as a potential therapeutic target in the treatment of these disorders.