Abcam, ab164770, Recombinant Human MBD3L1 protein

Size: 10µg
Recombinant Human MBD3L1 protein is a Human Full Length protein, in the 1 to 194 aa range, expressed in Wheat germ, suitable for ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q8WWY6,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.31% Glutathione

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MBD3L1 also known as Methyl-CpG Binding Domain Protein 3-Like 1 is a protein that functions by binding to methylated DNA regions. It has a molecular weight of approximately 28 kDa. This protein is expressed in several tissues with higher expression levels observed in testis and lymphocytes. MBD3L1 interacts with other proteins involved in chromatin remodeling and gene expression regulation. It does not possess a DNA-binding domain marking its diverse role compared to other members of the MBD family.
Biological function summary
MBD3L1 influences transcription regulation through its involvement in chromatin modification processes. It is part of a multiprotein complex that includes members like MBD2 and CHD4 which are known contributors to the nucleosome remodeling and deacetylase (NuRD) complex. This interaction facilitates the transcriptional repression activity and plays a role in establishing and maintaining DNA methylation patterns. MBD3L1's influence extends to important cellular processes such as cell differentiation.
Pathways
One can observe MBD3L1's involvement in the epigenetic regulation of gene expression and the DNA damage response pathway. The NuRD complex where MBD3L1 is a component regulates gene silencing important for epigenetic inheritance. Interaction with proteins like HDAC1 and MTA1 shows its role in histone deacetylation and chromatin remodeling. MBD3L1's activity ensures proper cell cycle progression and genomic stability impacting cellular responses to DNA damage.
MBD3L1 has been connected to cancer and autoimmune disorders. Abnormal expression of MBD3L1 and associated complexes can result in altered gene expression patterns contributing to oncogenesis. It associates with proteins like DNMT1 in cancer implying a role in aberrant DNA methylation patterns observed in tumorigenesis. In autoimmune disorders irregular chromatin modifications mediated by MBD3L1 complex can lead to improper immune cell development and function influencing disease progression.