Abcam, ab16640, Anti-Sortilin/NT3 antibody

Size: 100µg
Anti-Sortilin/NT3 antibody (ab16640) is a rabbit polyclonal antibody detecting Sortilin/NT3 in Western Blot . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Over 90 publications - Trusted since 2005
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-FoFrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-Sortilin/NT3 antibody (ab16640) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.
What is the molecular weight of Sortilin/NT3?
Anti-Sortilin/NT3 (ab16640) specifically detects a band for Sortilin/NT3 (UniProt: Q99523) at a molecular weight of 95kDa.
Trusted by the scientific community
Anti-Sortilin/NT3 (ab16640) was first used in a scientific publication in 2005 and has been cited over 90 times in peer-reviewed journals.
Reviewed by scientists
Anti-Sortilin/NT3 (ab16640) has over 10 independent reviews from customers.
Specificity confirmed
The specificity of Anti-Sortilin/NT3 antibody (ab16640) has been confirmed by Immunocytochemistry testing in Sortilin Knockout HAP1 cell line,
ab264772

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Sortilin also known as NT3 receptor is a type I membrane protein with an approximate molecular mass of 100 kDa. It is expressed in various tissues especially the central nervous system and adipose tissue. Sortilin belongs to the Vps10p domain receptor family and functions in intracellular protein sorting. Its primary role involves the transport and signaling of neurotrophins particularly NT-3 protein (neurotrophin-3) which is vital for neuronal growth and survival.
Biological function summary
NT-3 protein influences the development and differentiation of the nervous system. Sortilin serves as a receptor for NT-3 facilitating the interaction with the TrkC receptor on neuronal cells. Sortilin forms a complex with NT-3 contributing to critical cellular processes. Being part of this receptor complex Sortilin modulates neuron-related signaling pathways which are essential for neurogenesis and synaptic plasticity.
Pathways
Sortilin and NT-3 play significant roles in the neurotrophin signaling pathway and influence the MAPK signaling cascade. These pathways are pivotal in neuron survival differentiation and growth. NT-3 interacts with other proteins such as TrkC and p75 to propagate these signals. Through its association with TrkC receptor Sortilin mediates critical cellular responses linked to neurotrophin signaling. The interplay between these proteins allows precise regulation of neuronal activities in various physiological states.
NT-3 and Sortilin have associations with neurodegenerative conditions such as Alzheimer's disease and metabolic disorders like obesity. Dysregulation of Sortilin can contribute to abnormal NT-3 signaling affecting neuronal homeostasis. The protein also shows links with amyloid precursor protein (APP) in Alzheimer's disease where abnormal interactions may exacerbate disease progression. Understanding the dynamics of Sortilin in these conditions can offer insights into potential therapeutic approaches.