Abcam, ab166634, Anti-sPLA2-X antibody [EPR11202]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal sPLA2-X antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11202,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SPLA2-X also known as Group X secreted phospholipase A2 is an enzyme with a molecular mass of approximately 18 kDa. This enzyme catalyzes the hydrolysis of phospholipids at the sn-2 position yielding free fatty acids and lysophospholipids. sPLA2-X expresses mainly in human tissues including the airway epithelium which suggests its role in inflammatory responses and other physiological processes. This enzyme shows high affinity for certain membrane phospholipids indicating its potential interaction with cell membranes and involvement in lipid signaling.
Biological function summary
SPLA2-X contributes to the generation of bioactive lipid mediators such as eicosanoids which play key roles in cellular signaling. The enzyme does not act alone; it can associate with other proteins to form functional complexes that amplify inflammatory and immune responses. This activity becomes critical in host defenses and maintaining homeostasis during infections and injuries. Moreover by producing lipid mediators sPLA2-X influences processes like cell differentiation proliferation and apoptosis.
Pathways
SPLA2-X participates significantly in the arachidonic acid metabolism pathway leading to the production of pro-inflammatory mediators such as prostaglandins and leukotrienes. This enzyme interacts with cytosolic phospholipase A2 (cPLA2) and cyclooxygenases enhancing the generation of these mediators. Furthermore sPLA2-X also relates to the phospholipid-remodeling pathway affecting membrane dynamics and signaling. These interactions underline its significant role in modulating inflammation and other cellular processes.
SPLA2-X has been associated with conditions like asthma and rheumatoid arthritis where inflammation plays a central role. In asthma the enzyme's overactivity contributes to airway inflammation and hyperresponsiveness potentially through interactions with lung-related proteins such as leukotriene receptors. In rheumatoid arthritis sPLA2-X may exacerbate joint inflammation by enhancing the production of inflammatory mediators involving cytokines like tumor necrosis factor-alpha (TNF-α). Understanding these connections aids in targeting sPLA2-X for therapeutic interventions in such inflammatory disorders.