Abcam, ab16669, Anti-CD3 epsilon antibody [SP7]

Size: 20µL / 100µL / 500µL / 1mL
Anti-CD3 epsilon antibody [SP7] (ab16669) is a rabbit monoclonal antibody detecting CD3 epsilon in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, mIHC . Suitable for Human, Mouse, Rat . - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency - Over 720 publications - Trusted since 2005
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:SP7,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), mIHC, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-CD3 epsilon antibody [SP7] (ab16669) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), IHC-P, WB and mIHC.
Anti-CD3 epsilon antibody [SP7] (ab16669) was first used in a scientific publication in 2008 and has been cited over 722 times in peer reviewed journals. It's performance in IHC in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-CD3 epsilon antibody [SP7] (ab16669) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-CD3 epsilon antibody [SP7] (ab16669) has 79 independent reviews from customers.
Anti-CD3 epsilon antibody [SP7] (ab16669) specifically detects CD3 epsilon (UniProt ID: P07766; Molecular weight: 21kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL, 500 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone SP7 -
ab205228
Anti-CD3 epsilon antibody [SP7] (ab16669) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).
Top cited recombinant antibody on the market with >988 citations. SP7 has received >79 reviews. CD3 epsilon (CD3ε) is a crucial component of the T-cell receptor (TCR) complex. It plays a key role in T-cell activation and signal transduction by coupling antigen recognition to intracellular signalling pathways.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.1% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD3 epsilon also known as CD3e or CD3 epsilon protein is a component of the T-cell receptor (TCR) complex which plays a role in antigen recognition. The CD3 epsilon protein has a molecular mass of approximately 20 kDa. This protein is expressed in T cells across the thymus and peripheral lymphoid organs. It forms part of the CD3 complex which also includes CD3 gamma CD3 delta and CD3 zeta chains. This complex associates with the TCR alpha and beta chains facilitating critical signaling events for T-cell activation and development.
Biological function summary
CD3 epsilon functions as part of the TCR-CD3 complex essential for the immune response. It acts as a signal transducer initiating intracellular signaling upon antigen engagement. This signaling results in T-cell activation proliferation and differentiation. The CD3 complex including CD3 epsilon is a necessary participant in thymic selection a process ensuring the development of functional and self-tolerant T-cells. The specificity and function of CD3 epsilon make it a target for monoclonal antibodies such as anti-CD3 used for modulating T-cell functions.
Pathways
Several immune-regulatory pathways involve CD3 epsilon including the TCR signaling pathway and NF-κB pathway. CD3 epsilon works in conjunction with proteins such as CD28 and LCK to transmit signals from the TCR complex to downstream pathways. These pathways lead to the activation of transcription factors like NF-κB and AP-1 promoting cytokine production and T-cell proliferation. CD3 epsilon also interfaces with other pathways through interaction with zeta-chain-associated protein kinase 70 (ZAP-70) which is important for signal transduction.
Autoimmune diseases and certain types of cancer relate to CD3 epsilon activity. Aberrant functioning or expression levels of the CD3 epsilon protein can contribute to autoimmune conditions such as systemic lupus erythematosus due to improper T-cell activation. Additionally CD3 epsilon is targeted by therapeutic antibodies like muromonab used in prevention of acute organ transplant rejection. Altered pathways involving CD3 epsilon and associated proteins like CD3 zeta also have implications in leukemia highlighting the significance of this protein in both normal immune function and pathology.