Abcam, ab16700, Anti-Vimentin antibody [SP20]

Size: 20µL / 100µL / 500µL / 1mL
Anti-Vimentin antibody [SP20] (ab16700) is a rabbit monoclonal antibody detecting Vimentin in Western Blot, Flow Cytometry, IHC-P, ICC/IF . Suitable for Human . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 100 publications - Trusted since 2005
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:SP20,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WB, IHC-P, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:We have data to show that ab16700 is not suitable for work on mouse tissue. For researchers working on mouse we recommend using ab92547. If you would like further information on this, please do not hesitate to contact our technical support team.

Product details:
What is this antibody validated in?
Anti-Vimentin antibody [SP20] (ab16700) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of Vimentin?
Anti-Vimentin [SP20] (ab16700) specifically detects a band for Vimentin (UniProt: P08670) at a molecular weight of 53kDa.
Collaboration
Anti-Vimentin [SP20] (ab16700) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).
Trusted by the scientific community
Anti-Vimentin [SP20] (ab16700) was first used in a scientific publication in 2005 and has been cited over 100 times in peer-reviewed journals.
Reviewed by scientists
Anti-Vimentin [SP20] (ab16700) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Specificity confirmed
The specificity of Anti-Vimentin antibody [SP20] (ab16700) has been confirmed by Immunocytochemistry testing in VIM Knockout HAP1 cells.
Other related products
We have a range of other formats of antibody clone [SP20] also available for your convenience: ab16700,
ab27608
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.1% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle