Abcam, ab167718, Recombinant human HMGB1 protein

Size: 100µg
Recombinant human HMGB1 protein is a Human Full Length protein, in the 1 to 215 aa range with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE. The predicted molecular weight of ab167718 protein is 25.7 kDa.
Key facts
Purity:>95% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:HEK 293 cells,
Tags:His tag C-Terminus,
Applications:SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The EC50 for this effect is 0.7855‐0.8342 μg/ml (Routinely tested).,
Accession:P09429,
Animal free:No,
Carrier free:Yes,
Species:Human,
Reconstitution:It is recommended to reconstitute the lyophilized protein in 100µl sterile deionized water to a final concentration of 1mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.,
Storage buffer:pH: 7.4Constituents: PBS

Product details:
Ensure the validity of your result using our recombinant Human HMGB1 protein ab167718 as a positive control in SDS-PAGE.
The human HMGB1 protein has a calculated MW of 26.8 kDa. The ab167718 protein migrates at 32 kDa and 35 kDa under reducing (R) condition in SDS-PAGE due to glycosylation.
In functional ELISA the immobilized Human HMGB1 protein ab167718 at 5 μg/mL (100 µL/well) can bind Human RAGE, Fc Tag with a linear range of 0.078-2.5 µg/mL
Check out our protein gel staining guide for SDS-PAGE
Check out our ELISA protocol for more information

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle