Abcam, ab167740, Recombinant human SynCAM/CADM1 protein

Size: 100µg
Recombinant human SynCAM/CADM1 protein is a Human Fragment protein, in the 45 to 374 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, ELISA, FuncS.
Key facts
Purity:>95% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:HEK 293 cells,
Tags:His tag C-Terminus,
Applications:SDS-PAGE, FuncS, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Measured by its binding ability in a functional ELISA. Immobilized rhCADM1 at 2 µg/ml (100 µl/well) can bind biotinylated Human CRTAM with a linear range of 12.5 – 500 ng/ml.,
Accession:Q9BY67,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 100 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.,
Storage buffer:pH: 7.4Constituents: PBS, 5% Trehalose

Product details:
This product was previously labelled as SynCAM

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SynCAM also known as CADM1 (Cell Adhesion Molecule 1) is a protein that plays an important role in cell adhesion processes. It has an approximate mass of 105 kDa. SynCAM/CADM1 is expressed in various tissues including the brain where it supports synapse formation and maintenance. It is a member of the immunoglobulin superfamily and contains several I-set V-set and C2-set domains which contribute to its adhesion properties.
Biological function summary
SynCAM/CADM1 acts as a cell recognition molecule participating in the formation of synaptic connections in neural circuits. It is a component of a complex that regulates synaptic clustering and neural connectivity. In the nervous system SynCAM/CADM1 facilitates the initial axon-dendrite contact allowing efficient synaptic communication. Its expression and interaction affect the building of mature and functional synapses which are essential for cognitive processes.
Pathways
SynCAM/CADM1 integrates into synaptic development and cell signaling pathways. It interacts with scaffolding proteins like PSD-95 which stabilize the synaptic structure. The protein also participates in the Ras signaling pathway influencing cell growth and survival signals. Through its engagement in these pathways SynCAM/CADM1 plays a role in neural circuit plasticity and function maintaining proper communication between neurons.
SynCAM/CADM1 shows connections to neurological conditions such as schizophrenia and autism spectrum disorders. Its dysregulation is associated with abnormal neural connectivity leading to impaired cognitive function. SynCAM/CADM1's interaction with the L1CAM protein has been implicated in these disorders as both are involved in neural adhesion and signaling pathways that when altered can contribute to the development of such conditions.