Abcam, ab168727, Anti-c-Myc antibody [Y69] - ChIP Grade - BSA and Azide free

Size: 100µg / 1mg
Anti-c-Myc antibody [Y69] - ChIP Grade - BSA and Azide free (ab168727) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation. Suitable in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF, ChIP in Human, Mouse, Rat . - KO validated for confirmed specificity - BSA, sodium azide, and glycerol-free for easy conjugation - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:Y69,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), WB, ChIP-seq, IHC-P, ICC/IF, ChIC/CUT&RUN-seq, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody is specific for endogenous c-Myc. It does not detect Myc tag. Expression levels of the target protein vary with sample type and some optimization may be required.Please see documentation for further information on positive controls (Chinese version)

Product details:
What is this antibody validated in?
Anti-c-Myc antibody [Y69] - ChIP Grade - BSA and Azide free (ab168727) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP in Human, Mouse, Rat samples.
What is the molecular weight of c-Myc?
Anti-c-Myc [Y69] - ChIP Grade - BSA and Azide free (ab168727) specifically detects a band for c-Myc (UniProt: P01106) at a molecular weight of 48kDa.
Reviewed by scientists
Anti-c-Myc [Y69] - ChIP Grade - BSA and Azide free (ab168727) has over 10 independent reviews from customers.
Specificity confirmed
The specificity of Anti-c-Myc antibody [Y69] - ChIP Grade - BSA and Azide free (ab168727) has been confirmed by Flow Cytometry testing in MYC Knockout HEK-293 cell line,
ab256500
Other related products
We have a range of other formats of antibody clone [Y69] also available for your convenience:
ab32072
, Carrier free - ab168727, Alexa Fluor® 488 -
ab190026
, Alexa Fluor® 647 -
ab190560
, Alexa Fluor® 594 -
ab201775
, Alexa Fluor® 555 -
ab201780
, HRP -
ab205818
, FITC -
ab223913
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C-Myc also known simply as Myc or MYC protein is a transcription factor with significant roles in cellular processes. Its estimated molecular weight is approximately 62 kDa. This protein is expressed in various tissues and cell types including common use in HEK (human embryonic kidney) cells for research. c-Myc functions by regulating transcription of genes involved in cell cycle progression apoptosis and cellular transformation.
Biological function summary
C-Myc is involved in regulating cell growth and proliferation. It forms part of the Myc/Max complex which binds to DNA to regulate gene expression. This activity affects cellular metabolism ribosome biogenesis and cell cycle entry emphasizing its regulation of cellular energy and stress response. Its expression levels critically govern normal cellular functions and homeostasis.
Pathways
C-Myc plays an important role in the cell cycle pathway and apoptosis regulation. Specifically c-Myc is associated with the Wnt signaling pathway which influences cellular proliferation and differentiation. It also interacts with other proteins like Cyclin D1 influencing cell cycle control. These interactions ensure c-Myc's involvement in regulating key processes related to cell proliferation and stability.
C-Myc is tightly linked to cancer such as Burkitt's lymphoma and colon cancer. In these conditions c-Myc overexpression contributes to uncontrolled cell proliferation. Additionally c-Myc is associated with other oncogenic proteins like BCL2 in tumorigenesis highlighting its pivotal role in cancer development and progression. Understanding c-Myc's involvement in these diseases aids in the development of targeted therapeutic strategies.