Abcam, ab169083, Anti-NUSAP antibody

Size: 50µg
Mouse Polyclonal NUSAP antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NUSAP1 aa 200 to C-terminus.
Key facts
Host species:Mouse,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NUSAP1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9BXS6

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NUSAP1 also known as nucleolar spindle-associated protein 1 plays key role in cell division particularly in mitosis. It is located widely in the cell nucleus and gains its prominence during mitotic spindle assembly. With a molecular mass of about 55 kDa NUSAP1 is predominantly expressed in actively proliferating cells and has heightened expression in tissues with rapid cell division such as the thymus and bone marrow.
Biological function summary
NUSAP1 is significant in stabilizing microtubules and interacts with proteins like TPX2 to facilitate spindle formation. It binds to microtubules and augments their cross-linking and bundling influencing spindle dynamics. NUSAP1 does not work in isolation; it is a part of a larger complex that ensures proper chromosome segregation which is essential for accurate cell division.
Pathways
NUSAP1 is integral to the mitotic spindle assembly checkpoint an important part of the cell cycle. It associates with pathways such as the p53 signaling pathway which regulates cell division and growth. Within these pathways NUSAP1 interacts with proteins like AURKA and PLK1 marking its importance in maintaining DNA integrity and controlling mitotic events.
NUSAP1 has links to cancer especially in cases where cell proliferation is unregulated. Overexpression of NUSAP1 can lead to tumor progression making it a potential marker for cancer prognosis. Additionally disruptions in its function may tie to cell cycle disorders often connecting to proteins like MAD2L1 which also play roles in spindle checkpoint activities.