Abcam, ab16911, Anti-F4/80 antibody [BM8]

Size: 100µg
Anti-F4/80 antibody [BM8] (ab16911) is a rat monoclonal antibody detecting F4/80 in Flow Cytometry, IHC-Fr . Suitable for Human, Mouse . - Over 150 publications - Trusted since 2005
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:BM8,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-Fr, ICC, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Cell preparation containing Adgre1 protein. The exact immunogen used to generate this antibody is proprietary information.Q61549,
Specificity:The monoclonal antibody BM8 recognizes a 125 kDa extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. This antibody does not cross react with any of the following cell types from Mouse: granulocytes, mast cells, platelets, lymphocytes, fibroblasts or endothelial cells.

Product details:
Anti-F4/80 antibody [BM8] (ab16911) is a rat monoclonal antibody and is validated for use in Flow Cyt, ICC, IHC in mouse samples.
Anti-F4/80 antibody [BM8] (ab16911) has been cited over 152 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-F4/80 antibody [BM8] (ab16911) has high sensitivity and specificity.
Anti-F4/80 antibody [BM8] (ab16911) has 12 independent reviews from customers.
Anti-F4/80 antibody [BM8] (ab16911) specifically detects F4/80 (UniProt ID: Q14246; Molecular weight: 95kDa) and is sold in 100 µg selling sizes.
Antibody clone BM8 is also available pre-conjugated to a variety of labels for your convenience - Biotin, FITC, PE (
ab15694
ab60343
ab125148
F4/80 is a well-established marker for murine macrophages, playing a significant role in the immune system. It is involved in various immunological processes, including tissue homeostasis, inflammation, and immune responses. F4/80 is expressed on different macrophage subtypes, which can influence the progression of diseases such as autoimmune disorders, infections, and cancer. Its expression helps in identifying and isolating macrophages for research, aiding in the understanding of their roles in health and disease. It has been recently implicated as a key biomarker in some autoimmune diseases like IBD.
ab16911 is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation prcoesses in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell and diabetes in a mouse diabetes model.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Provided as a 0.2µm filtered antibody solution., Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.
Biological function summary
F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.
Pathways
F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.
F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.