Abcam, ab169385, Anti-MHF1 antibody

Size: 50µg
Mouse Polyclonal MHF1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CENPS.
Key facts
Host species:Mouse,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human CENPS.Q8N2Z9

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MHF1 also known as MHF complex subunit 1 stands for 'Molecular Happens Factor 1'. MHF1 acts as a girdle that helps in maintaining chromosomal stability. This protein is about 8 kDa in mass and gets expressed predominantly in the nucleus. It often interacts with other factors that ensure proper chromosome segregation and is involved in the repair of DNA double-strand breaks. You often see its presence in cell types with high proliferative ability as it supports important cellular division processes.
Biological function summary
MHF1 orchestrates several roles in maintaining genomic integrity. It functions as part of the MHF complex with CENP-S which is important in the centromere assembly process. The complex provides structural support and aids in recruiting other proteins essential for kinetochore formation. MHF1 through its interaction with such complexes enhances DNA repair mechanisms by facilitating homologous recombination. It ensures that cells can accurately repair DNA leading to reduced mutation rates and stable genotypes.
Pathways
MHF1 plays multiple roles in cellular processes. It is deeply involved in the DNA damage response pathway a critical sequence for detecting and repairing protein dysfunction. Within this pathway it works closely with proteins like RAD51 and MRN complex components. MHF1 also impacts the mitotic checkpoint pathway which is integral for proper chromosomal alignment and segregation. Together with kinetochores and involved proteins MHF1 ensures the cell cycle progresses without errors.
MHF1 holds significance due to its association with cancer and chromosomal instability syndromes. Faulty regulation or expression of MHF1 contributes to aberrant cell division leading to tumor growth. The protein's misfunction correlates with conditions such as ataxia-telangiectasia where it fails to repair DNA efficiently. In such cases MHF1 connects with ATM and BRCA1 important proteins that mediate DNA repair in response to damage underlying the disorder's progression.