Abcam, ab171095, Anti-CaMKII (phospho T286) antibody [22B1]

Size: 100µL
Anti-CaMKII (phospho T286) antibody [22B1] (ab171095) is a mouse monoclonal antibody detecting CaMKII in Western Blot, ICC/IF . Suitable for Human, Mouse, Rat . - Over 20 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:22B1,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Rat Calcium/calmodulin-dependent protein kinase type II subunit alpha phospho T286 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.P11275,
Specificity:ab171095 detects the phosphorylated alpha subunit CaMKII only. It does not detect the non-phosphorylated alpha subunit CaMKII or the ~60 kDa beta subunit in either phosphorylation state.

Product details:
What is this antibody validated in?
Anti-CaMKII (phospho T286) antibody [22B1] (ab171095) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of CaMKII?
Anti-CaMKII (phospho T286) [22B1] (ab171095) specifically detects a band for CaMKII (UniProt: P11275) at a molecular weight of 54kDa.
Trusted by the scientific community
Anti-CaMKII (phospho T286) [22B1] (ab171095) was first used in a scientific publication in 2013 and has been cited over 20 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CaMKII also known as calcium/calmodulin-dependent protein kinase II is an enzyme functioning critically in cellular signaling. It consists of multiple isoforms including alpha beta gamma and delta with the delta isoform Delta 1829 frequently highlighted for its specific roles. The molecular weight of CaMKII is approximately 50 to 60 kDa varying slightly between isoforms. CaMKII is widely expressed in the brain but is also present in the heart skeletal muscle and other tissues. Its structure includes a catalytic domain regulatory segment and association domain which allows auto-regulation and interaction with other molecules.
Biological function summary
CaMKII plays a vital role in cellular processes such as learning memory and muscle contraction. It can form a complex structure involving multiple subunits which allows for its unique ability to signal in response to calcium influx and calmodulin binding. This causes autophosphorylation of CaMKII allowing it to remain active even when calcium levels diminish. This property makes CaMKII essential for long-term potentiation (LTP) in neuron synapses influencing cognitive functions deeply.
Pathways
CaMKII is an important component of both the calcium signaling and synaptic plasticity pathways. In the calcium signaling pathway CaMKII interacts with calmodulin and other kinases to modulate calcium entry and signaling. In synaptic plasticity CaMKII works closely with proteins like NMDA receptors which regulate synaptic strength and modulation essential for encoding learning and memory. These interactions highlight its significance as a mediator between signaling cascades and cellular responses.
Abnormal regulation or expression of CaMKII links to Alzheimer's disease and cardiac arrhythmias. In Alzheimer's the dysregulation of CaMKII affects synaptic integrity and memory formation involving interaction with beta-amyloid plaques. In cardiac arrhythmias CaMKII dysfunction leads to improper cardiac muscle contractions often linked to the protein phospholamban. These associations highlight the clinical relevance of CaMKII in addressing and understanding mechanisms underpinning these diseases.